PCNA在大鼠肾上腺细胞培养中的表达及其意义

Expression and significance of PCNA in rats adrenocortical cell culture in vitro

目的:探讨增殖细胞核抗原(PCNA)在大鼠肾上腺细胞培养过程中的表达及其意义。方法:应用SP免疫组化法,检测PCNA在大鼠肾上腺细胞培养不同时期表达的强度变化,同时测定肾上腺细胞分泌皮质酮功能的动态变化。结果:在肾上腺细胞体外培养过程中,第3、5、7天,PCNA蛋白表达逐渐增强,分别为(18±3)%、(38±5)%、(70±6)%,第9、11天与第7天相比,PCNA蛋白表达减弱(P<0.05),皮质酮浓度测定显示在第7天皮质酮浓度达到最高峰(101.2±15.8nmol/L),随后逐渐下降,与PCNA蛋白表达强度的变化趋势一致。结论:肾上腺细胞体外培养在第7天肾上腺细胞增殖最旺盛,而且肾上腺分泌皮质酮达到最高峰。

Objective： To explore expression and significance of PCNA in rats adrenocortical cell culture in vitro. Metbods：Immunohistochemical methods were adopted, expressing strengths of PCNA were examined in ceil culture in different phase, meanwhile, corticosterone concentrations were dynamically measured. Results： In adrenocortical cell culture in vitro expressions of PCNA were increasing in the third, fifth or seventh day（ 18±3 %, 38 ±5%, 70±6% respectively）, in contrast to the ninth or eleven day PCNA gradually decreased（50±3%、39±2%, P 〈0.05）. corticosterone measurements showed corticosterone concentration was peak in the seventh day （101.2±15.8 nmol/L）, and gradually degraded. Changes of PCNA expressing strengths were consistent with those of corticosterone concentrations. Conclusions： Adrenocortical cells were the most proliferative in vitro and excreting corticosterone was at the most.