调节T细胞在梅毒患者中的表达及临床意义

Expression of Regulatory T Cells in Syphilis Patients

目的探讨梅毒患者外周血T细胞亚群及调节T细胞在梅毒病人发病中的临床意义。方法采用流式细胞术检测了32例梅毒患者以及30例健康体检者外周血T细胞亚群CD3、CD3+CD4+CD8-、CD3+CD4-CD8+、CD4+CD45RA+、CD4+CD29+、CD8+CD28+、CD8+CD28-、NK细胞以及CD3+CD4+CD8-和CD4+CD25+调节T细胞水平。结果与正常对照组相比,梅毒患者NK细胞的百分率明显降低(P<0.01),CD4+CD25+和CD3+CD4-CD8-调节T细胞的百分率明显升高(P<0.01),其它T细胞亚群无明显变化。结论梅毒患者NK细胞,CD4+CD25+和CD3+CD4-CD8-调节T细胞的异常变化可能是梅毒患者RPR持续阳性或临床症状反复,经久不愈的原因所在。

Objective To determine the percentage of T cell subsets and regnlatory T cells in peripheral blood of syphilis patients and the clinical significance of these T cell subsets. Method The percentage of CD3, CD3^＋CD4^＋CD8^-, CD3^＋CD4^-CD8^＋, CD4^＋CD45RA^＋, CD4^＋CD29^＋, CD8^＋CD28^＋, CD8^＋CD28^- T cell subsets, NK cells and CD4^＋CD25^＋ regnlatory T ceils in the peripheral blood of 32 patients with syphilis and 30 control subjects were determined by flow cytometry. Result Compared with the control subjects, the percentage of NK ceils in patients with syphilis was decreased （P 〈 0.05） and the percentage of CD4^＋CD25^＋ and CD3^＋CD4^-CD8^- regnlatory T ceils was significantly increased （P 〈 0.001 ）, and the percentage of other T cell subsets in syphilis patients was not changed.Condusion Abnormal changes of CD3^＋CD4^-CD8^- and CD4^＋CD25^＋ regulatory T ceils in syphilis patients may be responsible for the positive RPR test and the relapse of the disease.