牛碱性成纤维细胞生长因子cDNA的克隆及其酵母重组表达质粒的构建

Coloning of the cDNA conding for bbFGF and construction of express-ing plasmid pVT102U/a-bbFGF in the yeast Saccharomyces cerevisiae

根据已有资料设计并合成引物，利用ＲＴ－ＰＣＲ方法得到牛ｂＦＧＦｃＤＮＡ全序列，经过序列分析后，建立其酵母分泌型表达质粒ｐＶＴ１０２Ｕ／ａ－ｂｂＦＧＦ。

The fibroblast growth factors(FGFs)constittite a family of at least seven structurally related polypeptides,among them basic FGF(bFGF)is the most chracteristic.They play an important role in vivo in embryonic developement,angiogenesis,nervous cell system differentiation, and wound repair, According to the published cDNA sequeiice, the strtictural gene of bovine bFGF(bbFGF)was amplified by the RTPCR method, and Constructed in the expression plasmid pVT102U/a-bbFGF.