小鼠血液低剂量辐射刺激疗法提高机体抗化疗毒性反应的实验研究

Resistance to chemotherapeutic toxicity induced by blood low-dose irradiation stimulation treatment （BLIT）in mice.

目的 研究血液低剂量辐射刺激疗法（BLIT）防治化疗引起的骨髓造血干细胞损伤及小肠隐窝干细胞毒性反应的作用。方法 选用BALB/C小鼠，设BLIT合并化疗、单纯化疗二研究对照组，用脾克隆实验及小肠隐窝干细胞存活实验，观察化疗药物CBP引起的骨髓造血干细胞损伤及PDD引起的小肠毒性反应，计算BLIT的化疗防护系数，判断BLIT对化疗毒性反应的防护作用。结果 ①BLIT刺激回输合并化疗的脾克隆实验中，D016．035．Dq25．756；单纯CBP化疗组的D0为12．988、Dq为10．237；防护系数（PF值）为1．81，有显著的防护作用。QBLIT刺激回输合并化疗保护小鼠隐窝干细胞实验中，在PDD低剂量1mg／kg及高剂量9mg／kg剂量时均有明显的保护作用；在PDD3mg／kg、5mg／kg、7mg／kg时则无明显保护作用。结论 BLIT在化疗应用中可显著保护骨髓的造血干细胞，也可以保护小肠隐窝干细胞的化疗损伤，其机制很可能是BILT刺激回输启动了全身低剂量辐射刺激效应，使机体对化疗损伤产生了适应性反应，进而在某种程度上保护了机体的某些组织和器官。

AIM To study BLIT- induced resistance to chemo therapeutic toxicity in bone marrow haemopoietic stem cells and intestine crypt stem cells in mice, METHODS BALB/c mice were divided into groups receiving CBP or PPD only, or BLIT before CBP or PPD infusion. RESULTIn spleen clone experiment DO and Dq were 16.03,5 and 25.756 in group receiving BLIT, 12. 988 and 10.237 in group treated with CBP only with a RF 1.81. BLIT also exerted protection of intestine crypt stem cells against PDD at lrng/kg and 9mg/kg. CONCLUSIONBLIT can protect haemopoietic stern cells and intestine crypt stem cells from chemotherapeutic toxicity.