人胎脑纹状体发育过程中NSCs变化的原位杂交研究

Change of neural stem cells in striatuma from the developing human fetal brain at different stage: hybridization in situ

目的：观察人胎脑纹状体不同发育时期NSCs的变化。方法：60例胎龄16-28周的水囊引产胎儿，采用原位杂交及光镜技术对人胎脑纹状体NSCs的分布、形态以及数量进行检测。结果：不同胎龄人胎脑纹状体均可见Nestin mRNA阳性表达NSCs，呈圆形、椭圆形及星形等多形性，且明显群状分布，多数单个存在，细胞体积相差悬殊，有大有小，细胞有0-3个突起，胞核呈圆形和椭圆形，1-4个核仁不等，细胞染色质疏松。随着胎龄的增加，纹状体NSCs逐渐减少。结论：不同胎龄人胎脑纹状体均存在NSCs，NestinmRNA可作为定性NSCs的可靠标志物。

Objective：To observe the change of neural stem cells（NSCs） in striatuma during the developing human fetal brain at different fetal stage. Methods： 60 cases of embryoes at gestational 16w ～28w age and by induction of labor with water bag were collected for determining distribution, shapes, growth modes and the number of NSCs in striatuma with hybridization in situ under light microscope. Results：NSCs expressing Nestin mRNA existed in striatuma from human fetal brain at different stage and had obvious crowd distribution, most NSCs existed in a single growth mode, volumes of NSCs differ greatly, large or small. NSCs existed in striatuma of different fetal age included round cells ,oval cells and astrocytes, there were NSCs enations from 0 to 3. Nueli submitted round and oval shapes, each having nueleoli from 1 to 4. NSCs had rarefaction ehromatin, NSCs in striatuma gradually decreased with increase of age. Conclusions： NSCs exist in striatuma from different gestational stage, Nestin mRNA may be a reliable marker for determining NSCs.