艾恒与传统化疗药物在不同分化程度胃癌细胞株中敏感性的研究

Study of the sensitivity of the gastric cancer cell lines to oxaliplatin or common chemotherapy drugs

目的:探讨不同分化程度胃癌细胞对艾恒、5-FU、MMC和VP-16的敏感性。方法:将不同浓度的化疗药物与两株不同分化程度的胃癌细胞株共同温育,采用MTT方法检测化疗各药物对胃癌细胞增殖的影响。结果:四种化疗药物对两株胃癌细胞的增殖均具有显著的抑制作用,MKN45细胞株对各化疗药物敏感性顺序为:MMC>VP-16>艾恒>5-FU,而SGC7901细胞株对各化疗药物敏感性顺序为:MMC>艾恒>VP-16>5-FU。中分化的SGC7901对艾恒、MMC、VP-16的敏感性均显著高于低分化的MKN45,而MKN45对5-FU的敏感性高于SGC7901。结论:不同分化程度的胃癌细胞对药物的敏感性不同。

Objective： To investigate the sensitivity of Oxaliplatin, 5-FU,MMC and VP-16 in gastric cancer cell lines. Methods：Two gastric cancer cell lines with various degrees of differentiation were incubated with chemotherapy drugs of various concentration. The cytotoxicity was examined by MTr test. Results： All of Oxaliplatin, 5-FU, MMC and VP-16 had significant killing effect on human gastric cancer cells in vitro. Chemosensitivity sequence for single drug in poorly differentiated gastric cancer cell line MKN45 was MMC 〉 VP-16 〉 L-OHP 〉 5-FU, and that in moderately differentiated gastric cancer cell line SGC＇7901 was MMC 〉 L-OHP 〉 VP-16 〉 5-FU. SGC7901 is more sensitive to L-OHP, MMC, VP-16 but MKN45 is more sensitive to 5-FU. Conclusion： The effect of chemotherapy drugs on gastric cancer cells was different among 2 cemines.