用聚合酶链反应对天津市育龄妇女弓形虫病发病情况的研究

Incidence of Toxoplasmosis in Generative Women Investigated with PCR in Tianjin

随机选择1547例育龄妇女,其中妊娠妇女1266例,包括孕早期921例,孕中期345例:正常婚检妇女281例。另外有异常孕产史的非妊娠妇女133例,采用聚合酶链反应(PCR)检测血中TOX-DNA。阳性者、已流产者取其绒毛,未流产者待孕16周经B超定位取羊水进行TOX-DNA检测。结果:育龄妇女弓形虫病病人的发现率为4.52‰(7/1547),妊娠妇女的发现率为4.74‰(6/1266),非妊娠妇女为3.56‰(1/281),2例绒毛TOX-DNA阳性,1例羊水TOX-DNA阴性。有异常孕产史的妇女与正常妊娠妇女弓形虫病病人的发现率分别为50.56‰(9/178)和4.04‰(5/1237),具有统计学意义。

1 547 generative women (1 266 pregnant women involving 921 in early pregnancy stages and 345 in middle pregnancy stages,and 281 examined before marriage)and 133 non-pregnant women with abnormal pregnancy history as controls. All of the cases were studied for serum toxoplasma DNA(TOX-DNA) using the PCR technique. The tests were made for further evidence of TOX-DNA in the chorionic villus and amnionic fluid if blood TOX-DNA was positive. The results showed that the incidences of toxoplasmosis found in the generative women,pregnant women and non-pregnant women were 4. 52‰.(5/1 547),4- 74‰.(6/1 266) and 3. 56‰.(1/281),respectively. Two samples of chorionic villus tested for TOX-DNA were positive and three samples of amnionic fluids were TOX-DNA negative.. The incidence of toxoplasmosis found in the non-pregnant women with abnormal pregnancy history and normal pregnant women were 50. 56‰.(9/178) and 4. 04‰.(5/1 237) respectively,and there was a highly significant difference.