补肾活血复方对亚急性衰老大鼠慢性脑缺血模型细胞因子及抗凝因子的影响

Effects of bushenhuoxue complex on cytokine and anticoagulant factors in chronic cerebral circulation insufficiency model of subacute aging rats

目的:观察补肾活血复方对亚急性衰老大鼠慢性脑缺血模型细胞因子及抗凝因子的影响,并探讨其可能治疗机制。方法:健康雄性Wistar大鼠60只,随机分为正常组、模型组、治疗高剂量组、治疗低剂量组、对照组5组,每组12只。用D-半乳糖(D-gal)诱导亚急性衰老大鼠模型,在此基础上永久性结扎其双侧颈总动脉,制备衰老慢性脑缺血大鼠模型。观察补肾活血复方对模型大鼠脑组织形态学及血样中的超氧化物歧化酶(SOD)、丙二醛(MDA)、睾酮(T)、纤维蛋白原(Fib)、抗凝血酶Ⅲ(AT-Ⅲ)、内皮素(ET)、肿瘤坏死因子(TNF)、P-选择素(P-selectin)、一氧化氮合酶(NOS)等的影响。结果:模型组脑组织形态学呈缺血性改变,未见明显梗塞灶;模型组大鼠血清中SOD、NOS、T、AT-III降低(P<0.01),ET、TNF、MDA、Fib、P-selectin升高(P<0.01);补肾活血复方能升高SOD、NOS、T、AT-III(P<0.05),降低ET、TNF、MDA、Fib、P-selectin(P<0.01,P<0.05),且高剂量组(除MDA、ET外)优于低剂量组(P<0.01,P<0.05)。结论:补肾活血复方能纠正亚急性衰老大鼠慢性脑缺血后各种细胞因子及抗凝因子的失衡,其治疗机制可能是通过扩张脑血管,清除氧自由基,抗炎、抗凝等作用而多途径、多环节来实现的。

Objective： To observe the effect of bushenhuoxue complex on cytokine and anticoagulant factors in chronic cerebral circulation insufficiency model of subacute rats and to investigate the mechanism. Methods： Sixty healthy male Wistar rats were randomly divided into 6 groups： common, model, high-dose treatment, low-dose treatment, and control group. Subacute aging model was induced by D-galactose, and the bilateral arteria carotis communis was ligatured. The levels of SOD, MDA, T, Fib, AT-Ⅲ, ET, TNF, and P-selectin in plasma were observed. Results： The morphology of brain was ischemic changed and had no cerbral infarction in model group; The level of SOD, NOS, T, AT-Ⅲ dropped off（P 〈0.01） and of ET, TNF, MDA, Fib, and P-selectin rised（P 〈0.01） in model group, whereas the former fised （ P 〈 0.05） and the latter dropped off （ P 〈 0.01, P 〈 0.05 ） in therapy group; The effect for them in high-dose group was superior to low-dose group except for MDA and ET（ P 〈 0.01, P 〈 0.05）. Conclusion： Bushenhuoxue complex can correct the disequilibrium of cytokine and anticoagulant factors, which maybe by expanding the cerebral vessel, eliminating the free radical, inhibiting the inflammation and collecting the platelet.