Hp感染及COX-2的表达与胃癌及癌前病变关系的研究

Correlation between Hp infection and expression of COX-2 in gastric cancer and precancerous lesion

目的:探讨幽门螺杆菌(helicobacter pylori,Hp)感染的胃癌及癌前病变患者环氧化酶2(COX-2)的表达及Hp与COX-2协同作用与胃癌发生的关系。方法:应用免疫组化方法测定62例COX-2表达情况,选择20例胃癌、30例萎缩性胃炎(其中包括18例异型增生及12例肠上皮化生)、12例浅表性胃炎,用快速尿素酶法和HE染色检测Hp感染情况。结果:胃癌组Hp感染12例(12/20,60%)。COX-2在异型增生组织中的阳性表达率与胃癌、肠上皮化生相比差异无统计学意义(P>0.05)。Hp阳性组肠上皮化生、异型增生组织及胃癌中COX-2的阳性表达率显著高于Hp阴性组(P<0.05)。结论:Hp菌株感染可上调胃癌组织中COX-2的表达,Hp感染和COX-2过度表达在胃癌的发生中起重要作用。

Objective： To investigate the possible role of Helicobacter pylori （Hp） infection in the development of gastric cancer. Method： Immunohistochemical staining was used to detect the expression of COX-2 in 12 cases of chronic superficial gastritis, 30 cases of chronic atrophic gastritis （including 12 cases of intestinal meta- plasia and 18 cases of dysplasia ）and 20 cases of gastric cancer. Hp was assessed by using rapid urease staining test combined with HE staining test. Results： The infection of HP in gastric carcinoma was found in 12 cases （12/20, 60% ）, and there was no significant difference in the positive rate of COX-2 between dysplasia and gastric cancer or intestinal metaplasia（ P 〉 0.05）, and their expression level were intensive in Hp positive patients when compared with Hp negative patients in precancerous lesions （ P 〈 0.05）. Conclusion： COX-2 gene expression is accelerated in early gastric cancer. Hp might act as a tumor promoter in the genesis of gastric carcinoma.