卵巢癌患者血清及组织HGF测定和受体c-Met的表达与预后关系的研究

Expression of hepatocyte growth factor and c-met receptor in ovarian epithelial tumors

目的:探讨血清肝细胞生长因子(hepatocyte growth factor,HGF)在卵巢癌中的诊断价值和HGF及其受体c-Met表达与其临床病理特征及预后的关系。方法:酶联免疫吸附法(ELISA)测定67例上皮性卵巢癌、25例良性上皮性肿瘤、30例健康对照者血清HGF含量;免疫组织化学法检测肿瘤组织中HGF及c-Met蛋白表达。结果:①卵巢恶性上皮性肿瘤组术前血清HGF水平显著高于良性上皮性肿瘤组和正常对照组(P<0.05);②卵巢恶性上皮性肿瘤患者术前血清HGF水平与其临床分期、分化程度、淋巴结转移、术后残存灶的大小有关(P<0.05),与肿瘤组织学类型无关;③HGF与c-Met在卵巢良恶性上皮性肿瘤组织间的表达有显著性差异;④卵巢恶性上皮性肿瘤组织中HGF表达与肿瘤的组织学类型无关,与临床分期、分化程度、淋巴结转移有关;c-Met表达与组织学类型、临床分期无关,与分化程度及淋巴结转移有关。结论:HGF可用于卵巢恶性肿瘤诊断和术后随访的肿瘤标记物,与卵巢恶性上皮性肿瘤的发生、发展相关,HGF与c-Met联合检测对于判断卵巢恶性上皮性肿瘤预后有重要意义。

Objective： To explore the clinical value of the serum content of HGF in ovarian epithelial carcinomas, also to explore the relationship between HGF, c-Met protein expression and the clinical pathological figures in patients with ovarian epithelial carcinomas. Methods： Serum samples from 67 ovarian epithelial carcinomas, 25 benign epithelial tumors and 30 healthy volunteers were examined the HGF content by the quantitative ELISA, and the HGF and its receptor c-Met protein expression by immunohistochemistry. Results： Firstly, the HGF levels before surgery in ovarian epithelial carcinomas was significantly higher than those in ovarian benign tumors and control group （P 〈0.05）. Secondly, HGF levels had significant association with tumor stage, histological grade, lymph node metastasis, and residual lesion before surgery, but not with histological type. Thirdly, the expression of HGF and c-Met protein in ovarian epithelial carcinomas were significantly higher than that in benign epithelial tumors. Fourthly, there was significant association of HGF levels with clinical stage, histological grade, lymph node metastasis in ovarian epithelial carcinomas, but not with histological type. Conclusions： HGF may be a new useful tumor marker for screening and monitoring malignant tumor patients, and its expression is correlated with the carcinogenesis and progress of ovarian epithelial carcinoma, both HGF and c-Met may be significant prognostic factors of ovarian epithelial carcinoma.