高寒藏药──川西獐牙菜组织培养研究Ⅰ.愈伤组织的诱导及初步培养

Tissue Culture of Chuanxizhangyacai(Swertia mussotii)Ⅰ.Induction and Maintenance of the Callus

从川西獐牙菜的胚轴、未成熟种子及幼叶诱导出愈伤组织；试验选用MS、B5和N63种培养基，其中以加有2，4－D3mg／L＋KT0.6mg／L的MS培养基诱导率最高。结果表明,不同培养基及不同激素组合对愈伤组织诱导率有较明显的影响。初步证明了用植物组织培养的方法进行川西獐牙菜的无性快速繁殖是有可能的。

Calli were induced from young leaves,immature seeds and hypocotyl of Swertia mussotii Franch.Three kinds of media,MS,B5 and N6 were set up in the experiment. Calli were established with the highest frequency on to MS medium cantaining 3mg/L 2,4-D and 0. 6mg/L KT.The induction antmaintenence of the calli were affected markedly by the basic media and compound 2,4-D and KT in the media which proved that asextual reproduction of S. mussotii is possible by tissue culture