近红外反射光谱分析技术快速检测复合化学肥料中肉骨粉含量的研究

RAPID DETECTION OF MEAT AND BONE MEAL CONTENT IN COMPOUND FERTILIZER BY NEAR INFRARED REFLECTANCE SPECTROSCOPY

肉骨粉是产生牛海绵状脑病(BSE)的主要原因,根治BSE很有必要研究饲料和肥料中的肉骨粉的检出方法.本文探讨了利用近红外反射光谱(NIRS)分析技术快速检测复合化学肥料中肉骨粉含量的可行性.对混合有肉骨粉的4种复合肥料样品和这些所有样品,采用偏最小二乘法(PLS),获得5个校正模型的决定系数(R2)分别为0.9989、0.9957、0.9977、0.9969和0.9955,相对标准偏差(RSD)分别为3.82%、4.07%、3.92%、3.99%和5.13%;检验集的真值和模型预测值的决定系数(r2)分别为0.9951、0.9981、0.9960、0.9943和0.9884,RSD分别为6.74%、2.58%、3.55%、6.17%和7.44%.结果表明,利用NIRS分析技术可以成功检测出复合肥料中肉骨粉的含量.

Bovino spongiform encephalopathy（BSE） is mainly caused by meat and bone meal（MBM）. In order to eliminate BSE, it is imperative to develop a method to examine the content of MBM mixed in feedings and fertilizers. The objective of this study was to investigate the feasibility of using near infrared reflectance spectroscopy （NIRS） to determine MBM content in compound fertilizers. By partial least squares （PLS） regression, 5 NIRS models were developed from 5 calibration sets, which were of mixed samples of 4 various compound fertilizers with MBM and their collectivity. The determination coefficient （ R^2 ） of 5 calibration sets was, respectively, 0. 9989, 0. 9957, 0. 9977, 0. 9969, 0. 9955, and the relative standard deviation（RSD） was, respectively, 3.82%, 4.07%, 3.92%, 3.99%, 5. 13%. The coefficient of determination （r^2） of independent validation sets was 0. 9951, 0. 9981, 0. 9960, 0. 9943, 0. 9884 respectively, and the RSD was 6.74%, 2.58%, 3.55%, 6.17%, 7.44% respectively. It is concluded that NIRS can be used as a method to detect MBM content in compound fertilizer.