铬在大鼠急性肺损伤中变化及维生素C的干预作用

The Infuluence of Vitamin C Effects on Chrome Level to the Rat when ALI

目的了解大鼠急性肺损伤(ALI)铬水平的变化及维生素C对其的影响。方法将ALI实验动物随机分为12组,其中6组为维生素C治疗组,ALI后均立即给予维生素C治疗,依照采集肺组织标本不同时间点分为:6 h组、8 h组、12 h组、16 h组和24 h组(均12 h追加一次维生素C)、36 h组(12和24 h各追加一次维生素C);用生理盐水替换维生素C为6组对照组。用原子吸收分光光度计检测肺组织铬含量。结果治疗组组间、对照组组间有明显差异(P<0.05);各时间点治疗组与对照组差异明显(P<0.05或P<0.01)。除治疗6 h组外,治疗组均高于对照组。结论维生素C可提高大鼠ALI时肺组织铬含量。

Objective To study the change of the Chrome level, on the rats suffered ALl, and the infuluence of Vitamin C effects on them. Methods Divide the rats into 12 sets, 6 sets among which are therapied by Vitamin C （give all of them Vitamin C immediately after ALI）, while substitute the Vitamin C by NS in the other 6 sets. In addition, to distinguish the therapied sets according to the different time point of the sample （the tissue of lung） as： 6 h set,8 h set,12 h set,16 h set and 24 h set （all annex Vitamin C for just one time per 12 hours） ,and 36 h set （annex Vitamin C for twice per 12hours） ; the NS-substi tuted sets are distinguished in the same way. Then, use the atom spectrophotometer to detect the content of Chrome in the samples, Results Among the therapied sets and comparison sets show the significant change （ P 〈 0.05） ; therapied sets and comparison sets in each time show the significant change （P〈0.05 或 P〈0.01 ）; except 6h, therapied sets are higher than comparison sets. Conclusions The level of Chrome step up transiently by Vitamin C when ALl in lungs of rat.