两种方法测定二氧化碳的应用评价

Evaluation of Two Methods for the Detection of CO_2

目的比较磷酸烯醇式丙酮酸羧化酶（PECE）与碳酸酐酶（CA）测定血浆中的二氧化碳的优缺点。方法以混合血浆为标样，用PECE及CA分别测定高、中、低值二氧化碳；同时随机取临床标本50份作对比实验。结果精密度显示PECE天内二氧化碳CV为1．56％～1．92％，天间CV为2．85％～3．78％；CA天内二氧化碳CV为3．89％～5．58％．天间CV为4．23％～6．75％：PECE回收率在96．7％～102．6％，而CA在89．4％～91．9％；PECE线性试验结果相关系数为0．9973；CA线性试验结果相关系数为0．9829。PECE与CA对比测定结果相关系数为0．9918。结论PECE操作简便、精确：快速、重复性、回收率、线性及相关性好等特点，用血量少，适用于全自动生化分析仪。

Objective To compare Phosphoenolpyruvate Carboxylases （PECE） method with Carbonic Anhydrase （CA） method for detection of plasma CO2. Methods Mixed plasma used as control, and high, medium and low value of CO2 were determined by PECE method and CA method, respectively. Simultaneously, fifty clinical samples were collected at random and detected as comparative experiment. Results Collected of variation （CV） of CO2 in PECE method was 1.56-1.92% in the same day and 2.85 - 3.78 % in day to day. CV of CO2 in CA method was 3.89 % - 5.58 % in the same day and 4.23 % - 6. 75 % day to day. Recovery rate of PECE method was 96.7% - 102.0% while that of CA method was 89.4% -91.9%. Correlation coefficient of their results was 0. 9973 while that of CA method was 0. 9829. Correlation coefficient of their results was 0.9918 when PECE method was compared with CA method. Conclusions PECE method is more convenient, precise and there are good repetitiveness, recovery rate, linearity and correlation when compared with CA method. Moreover, PECE method can be used in automatic biochemical analyser because of less consumption of plasma.