肝郁证、脾虚证、肝郁脾虚证下丘脑、蓝斑CRF含量变化研究

Contents of Corticotropin Releasing Factor in Hypothalamus and Locus Ceruleus in Rats with Liver-Depression Syndrome, Spleen-Deficiency Syndrome and Liver-Depression and Spleen-Deficiency Syndrome

目的探讨肝郁脾虚证、脾虚证、肝郁证与神经肽CRF之间的相关性。方法用足电刺激、大黄灌胃、电刺激+大黄灌胃方法分别复制肝郁证、脾虚证、肝郁脾虚证大鼠模型,用放射免疫方法测定各组实验大鼠下丘脑和蓝斑CRF含量。结果肝郁组下丘脑CRF含量降低,脾虚组下丘脑CRF含量存在升高趋势,二者之间有显著性差异(P<0.05);肝郁组蓝斑CRF含量明显升高,与正常组、脾虚组、肝郁脾虚组比较均有显著性差异(P<0.05)。结论肝郁组下丘脑CRF降低,脾虚组CRF在下丘脑存在升高趋势;肝郁组蓝斑CRF明显升高。下丘脑CRF在肝郁组和脾虚组之间的差异,可能是肝郁证和脾虚证的中枢差异点之一;肝郁组蓝斑CRF升高,可能是肝郁证证本质的核心点。

Objective To study the correlativity between liver-depression and spleen-deficlency syndrome, spleen-deficiency syndrome, liver-depression syndrome and corticotrophin releasing factor（CRF）. Methods Corresponding rat models were established by electro-acupuncture and gastric perfusion of Rhubarb. Radioimmunoassay was adopted to detect the contents of CRF in hypothalamus and locus ceruleus. Results CRF in hypothalamus fell in liver-depression rats, while it rose in spleen-deficiency syndrome, P〈 0.05; CRF in locus ceruleus rose in liver-depression syndrome, with a significant difference in comparison with other syndromes, P 〈 0.05. Conclusion CRF may be the core of the essence of liver-depression syndrome.