平调饮对小鼠S180骨肉瘤细胞PCNA、Ki-67、EGF及EGFR表达的实验研究

Experimental research of Pingtiaoyin on presented and expressed of PCNA、Ki-67、EGF and EGFR in S180 osteosarcoma mouse

目的:探讨平调饮对小鼠S180骨肉瘤细胞PCNA(增殖细胞核抗原)、Ki-67(肿瘤增殖抗原)、EGF(表皮生长因子)及EGFR(表皮生长因于受体)表达的影响及抑瘤率。方法:观测平调饮低剂量组、高剂量组及西药对照组对荷瘤小鼠的抑瘤率,运用免疫组化方法观察四种因子在实验小鼠肿瘤组织中的表达率。结果:中药低剂量组、高剂量组、西药对照组对荷瘤小鼠的抑瘤率分别为32.10%、43.97%和45.90%。中药低剂量组、高剂量组、西药对照组均能降低四种因子在实验小鼠肿瘤组织中的表达率。结论:平调饮对荷瘤小鼠肿瘤组织有抑制作用,能降低四种因子的表达率。

Objective：To discuss the Pingtiaoyin on presented and expressed of PCNA, Ki- 67, EGF and EGFR in S180 esteosarcoma mouse,and its antimmor rates.nethods：Severul kinds of immune factors of esteosarcoma are presented and expressed highly. The more outsanding ones is Ki - 67, PCNA, EGF and EGFR. The author carried on the literature survey on these factors and the relation and the meanings in their expression of esteosarcoma, and has also carried on resarch to the therapy of combining traditional Chinese and Western medicine of osteosareoma. Results： The experiment research has identified the treatments that Pingtiaoyin high dosage and low dosage group operated on S180 Osteosarcoma mouse,then I compare the results with the blank antitheses group and the western medicine antitheses group.I also observe the expressed rate of Ki - 67, PCNA, EGF and EGFR in the groups using immune mehod. The antitumor rate of traditional Chinese medicine low dosage group, high dosage group and Western medicine antitheses group to S180 esteosarcoma mouse are 32.10 %, 43.97 % and 45.90 %. There are prominent differences among the groups （ P 〈 0.05 ）. Conclusion：It has identified that traditional Chinese medicine Pingtiaoyin has great function on the esteosareoma S180.It can lighten the poisonous side reaction of the chemotherapy medicine, improve the immunological function of the little mouse and survival quality.