培养法检测小儿肺炎支原体的临床价值

Diagnosis Value of Culture Detection of Mycoplasma Pneumoniae

【目的】评价培养法检测肺炎支原体(MP)的性能,了解肺炎支原体的耐药状况,指导临床合理用药。【方法】采用酶联免疫吸附(ELISA)法检测肺炎支原体IgM抗体和培养支原体检测146例小儿咳嗽患者。【结果】培养法检测MP阳性率明显高于ELISA法(P<0.01)。肺炎支原体对克拉霉素耐药率最高,占58.43%,对环丙沙星和司帕沙星耐药率为0%。【结论】培养法是检测MP感染的简便有效的方法,并且能提供药敏结果。

[Objective]To estimate the capability of culture detection of mycoplasma pneumoniae and acquire the information about the present resistance characteristics in MP, and to instruct reasonable application of antibiotics clinically. [Methods]ELISA and mycoplasma culture were used to detect MP in clinical samples obtained from 146 children with cough. [Results]The positive rate of detection was significantly higher with culture than with ELISA （P〈0.01）, The result of susceptibility showed that the highest resistant rate was to clarithromycin （58.43%）, and were sensitive to ciprofloxacin and spaxacin. [Conclusion]Mycoplasma culture method is highly applicable in clinical laboratories for the detection and diagnosis of mycoplasmal infections, and drug sensitive results can be obtained.