川芎嗪联合顺铂治疗Lewis肺癌的实验研究

Experimental Research on TMP Combination with DDP in Treating Lewis Lung Carcinoma

【目的】观察川芎嗪联合顺铂对Lewis肺癌小鼠移植瘤生长的抑制作用。【方法】复制Lewis肺癌小鼠移植瘤模型,将40只接种Lewis肺癌细胞C57BL/6小鼠随机分成4组:对照组、顺铂组(DDP)、川芎嗪组(TMP)、联合组(DDP+TMP)。治疗期间,观察小鼠的毒副反应以及生存质量。实验19 d后,处死全部小鼠,剥离皮下肿瘤,称小鼠肿瘤瘤重量,计算出抑瘤率以及肿瘤坏死情况。【结果】顺铂组、川芎嗪组、联合组与对照组相比肿瘤抑制率升高,联合治疗组小鼠抑瘤率明显高于川芎嗪组(P<0.01)与顺铂组(P<0.01)。联合治疗组肿瘤坏死率明显高于顺铂组(P<0.05),与川芎嗪组比较,差异无显著性(P>0.05)。联合治疗组小鼠毒副反应明显低于顺铂组,生存质量优于顺铂组。【结论】川芎嗪和顺铂对Lewis肺癌小鼠移植瘤的生长具有协同抑制作用,并能降低毒副反应,提高生存质量。

[Objective]To explore the inhibition action induced by Tetramethylpyrazin（TMP） combined with cisplatin （DDP） on Lewis lung carcinoma in mice. [Methods]Lewis lung cancer cells were inoculated into the mammary fatty pad of C57BL/6 mice to establish Lewis lung carcinoma model,40C57BL/6 mice bearing highly metastatic Lewis lung cancer cells were randomized into four groups： control group, DDP group, TMP group, TMP ＋DDP group. Different treatments were served from day 7 after transplantation and all mice were sacrificed after 19 days. During the treatmenf, the toxic side reaction of chemotherapy and the life quality were observed. Subcutaneous tumors were processed for tumor weight, and tumor growth inhibition rate and tumor necrosis rate of each group were calculated. [Results]In every treatment group tumor growth was suppressed significantly, intraperitoneal injection of DDP,TMP and their combination resulted in a significant inhibition on the growth of Lewis lung cancer cells in vivo（ P〈0. 01）, the combination group showed significant enhancement in anti-tumor efficacy. Compared with DDP group, the tumor necrosis rate in combination group was significantly higher （ P 〈 0. 05 ）, but not remarkably in TMP group （ P〉0. 05 ）. Compared with DDP group, the toxic side reaction of chemotherapy in combination group was significantly lower, but life quality higher. [Conclusion] Combined application of Tetramethylpyrazin and Cisplatin has stronger inhibition on tumor growth than single use, and they have a synergic anticancer effect to Lewis lung cancer in mice, decrease toxic side reaction of chemotherapy and improve life quality.