中药治疗大鼠去卵巢骨质疏松症的蛋白质组学分析

Bone Proteomic Analysis About Chinese Medicine Action On Rat Ovariectomy Model of Osteoporosis.

目的:用蛋白质组学的方法对绝经后骨质疏松症和中药作用机理进行研究,有利于临床防治该疾病。方法:建立去卵巢大鼠骨质疏松症模型,设立中药治疗组及假手术对照组,6周后骨质疏松症模型成功,行骨形态学检查,提取骨组织蛋白质样品,双向电泳分离,得到各组骨组织总蛋白质分子解剖图谱,用图像分析软件,分析各组间差异蛋白质点,MADITOF/MS质谱分析,结合蛋白质生物信息库(Matrix science Ltd database),对各蛋白质初步鉴定。结论:鉴定了3个差异蛋白,分别为P1硫氧还蛋白过氧化酶1(Thioredoxin peroxidase 1),P2为阻凝蛋白轻链肽2(Myosin light polypeptide 2),P3为泛素化酶E2-17KD(ubiquitin-conjugating enzyme E2-17kD)。初步认为这三种蛋白质在绝经后骨质疏松症的发病及中药治疗过程中发挥着重要调控作用。

Objective： To study the mechanism about osteoporosos of OVX （ovariectomy） and Chinese Medicine action on osteoporosis in proteomics, it is of benefit to prevent and cure this disease. Methods ： Bilateal OVX in rats was performed as osteoporosis model. Rat was randomly divided into three groups： control group, osteoporosis model group and Chinese Medicine therapy group. The pathology of bone was examined after 6 weeks. In the present proteomic study, we characterized the protential effects of OVX and Chinese Medicine on protein expression in rat bones. Using two-dimensional gel electrophoresis, mass spectrometry, and Matrix science Ltd database, we elementarily identified three variational proteins.Conclusions： Three proteins were identified as proteins similar to thioredoxin peroxidase 1, Myosin light polypeptide 2 and ubiquitin-conjngating enzyme E2-17kD.These proteins have been demonstrated to be postmenopausal proteins. These results can provide valuable experimental evidences for the research for the molecular mechanism of osteoporosis which was response to OVX and Chinese Medicine in bone.