去垂体对壮医药线点灸调节脾虚大鼠生长抑素的影响

Effect of Medicated Thread Moxibustion of Zhuang Traditional Medicine on SS of Pituitaryectomied Rat in Experimental Spleen Deficiency

目的:观察去垂体后壮医药线点灸对实验性脾虚大鼠下丘脑、胃、肠生长抑素(Somatostatin,SS)的影响,探讨壮医药线点灸治疗脾虚的机理。方法:用耗气破气加饥饱失常法建立100只实验性脾虚大鼠模型,经耳摘除脑垂体。放射免疫分析法检测壮医药线点灸治疗前后下丘脑、血、胃、肠组织中SS的含量,并设立空线点灸对照、中药四君子汤治疗对照、模型假手术对照、模型自然恢复对照及正常健康对照,对比分析各组的变化。结果:脾虚大鼠去脑垂体后,下丘脑、胃、肠组织中的SS含量明显高于同期正常健康对照和垂体保留模型对照(P<0.01);血中的SS含量低于同期正常健康对照和垂体保留模型对照(P<0.01)。壮医药线点灸治疗后,除下丘脑的SS含量略有下降外,胃、肠、血中的SS含量没有明显变化,治疗后与去垂体模型组比较无显著性差异(P>0.05);中药组与去垂体模型比较有显著性差异(P<0.01)。结论:脾虚大鼠去垂体后,壮医药线点灸对下丘脑、胃、肠、血SS调整作用不明显,推论壮医药线点灸调节SS的效应机制与垂体有关。

Objective： To explore the mechanism and effect of Medicated Thread Moxibustion of Zhuang Traditional Medicine （MTMZ） on SS of pituitaryectomied rats in experimental spleen deficiency. Methods： 100 model rats of spleen deficiency were estabfished by exhaustion, dissipating stagnant qi and irregular diet, then pituitaryectomied, as compared with the control group. Contents of SS in hypothalamus, stomach, intestines and blood were determined by radioimmuno-assay （RIA） and their changes were studied before and after treatment. Results： The SS levels of hypothalamus, stomach, intestines in the pituitaryectomied rats of spleen deficiency were higher than those of the normal control and model group with pituitary （ P 〈 0.01） .The SS levels of blood in the pituitaryectomied rats of spleen deficiency were lower than those in the normal control and model group with pituitary P 〈 0.01. After treatment of MTMZ, there were not remarkably changes of the SS levels in the hypothalamas, blood, stomach and intestines as compared with model group of pituitaryectomied （ P 〉 0.05）, and there were remarkably changes of the SS levels in Si Jnn Zi decoction （SJZD） group as compared with model group of pituitaryectomied （ P 〈 0.01 ） . Conclusions ： MTMZ has no regulatory action on SS in hypothalamu、stomach、 intestines of pituitaryectomied rats with experimental spleen deficiency.