中药对软组织创伤愈合受体酪氨酸激酶活性的影响

Effects of TCM Herbs on the activity of receptor tyrosine kinase in wound of soft tissue in mice.

目的:探讨中药对软组织创伤愈合RTK活性变化的影响。方法:昆明种小鼠,制作软组织创伤模型,口服中药为实验组,未用中药为对照组。术后分别于第2、3、5、7、10天处死动物,采用膜结合法测定伤后2、3、5、7、10天创伤组织中RTK活性变化。结果:实验组创伤组织中RTK活性均高于对照组(P<0.01或P<0.05),RTK活性创伤愈合第10天接近正常水平。结论:中药可以增强软组织创伤愈合中的RTK活性。

Objective： To elucidate the changes of activity of receptor tyrusine kinase （RTK） in wound of soft tissue in mice after using Chinese Traditional Medicine Herbs （TCMH） . Methods： Experimental trauma models of soft tissue were made on Kunming mice, those who given oral TCMH as the experimental groups and without TCMH as control group. The mice were sacrifuced successively at 2nd, 3rd, 5th, 7th, 10th day after operation.The RTK activity in the wound were measured by using menbranous conjugated method. Results： The RTK activity in wound tissue of the experimental group was obviously elevated as compared with that of control group from 2nd day to 7th day （ P 〈 0.01 ） . RTK activity of wound tissue got near to the level of normal soft tissue at 10th day after operation. Conclusions： The TCMH could promote the activity of RTK of wound of soft tissue in mice.