布氏田鼠胎后发育过程中褐色脂肪组织和肝脏的产热特征

Changes in thermogenic properties of brown adipose tissue and liver during postnatal development in Brandt’s voles (Lasiopodomys brandtii)

为探讨布氏田鼠胎后恒温能力的发育过程,本文测定了1、5、9、17、21、33和41日龄幼体的褐色脂肪组织(BAT)和肝脏的重量、线粒体蛋白含量和细胞色素c氧化酶(COX)的活性。布氏田鼠胎后发育期间BAT增补明显,主要表现为重量的增加和单位组织重量COX活性的升高等,属典型的晚成型发育特征。布氏田鼠胎后发育过程中BAT和肝脏产热特征的变化与幼体的产热特点和恒温能力的发育是相一致的。

To understand the postnatal development of thermoregulatory ability in Brandt ＇ s voles （ Lasiopodomys brandtii ） , we measured the mass as well as mitochondrial protein contents and eytochrome c oxidase （COX） activity of brown adipose tissue （BAT） and liver at day l, 5, 9, 17, 21, 33, and 41 respectively. BAT mass and COX activity （per g tissue） during postnatal development increased markedly. On day 5, COX activity （nmolO2 · min^-1· g^-1 tissue） in BAT （923. 11 ±45.47） and liver （728. 40 ±28.52） was significantly higher than day 1 （718.40 ±28.8 and 651.56 ± 13.95, respectively）. Our data show that postnatal changes ill thermogenic properties in BAT and liver mitochondrian are consistent with their thermogenic ability in Brandt＇ s voles.