摘要
采用金葡菌A蛋白标记辣根过氧化酶(HRP-SPA)免疫组化方法,观察62例胃癌切片和94例胃涂片癌胚抗原(CEA)反应。结果显示;CEA存在于胃癌细胞及胃粘膜上皮细胞内,具有极性分布特征;细胞分光光度计(CSM)检测,胃癌细胞内CEA含量显著高于胃上皮细胞,且与腺癌分化程度有关;胃涂片细胞CEA阳性率高于胃癌病理检出率,二者符合确诊率亦较高。提示:CEA免疫组化检测可辅助胃癌病理学早期诊断及确定分化程度。
The HRP-SPA immunohistochemical technique was used to observe Presence of CEA(carcinoembryonic antigen) in Paraffin sections of 62 stomach cancer specimens and 94 endoscopic gastric smears.CEA weak positive was found both in the cytoplasm of normal columnar or goblet-cells with polarizing distribution.CEA positivity reaction was detected by cytospectrophotometer in adenocarcinoma tissue with greater intensity than that in normal stomach tissue.There was an obvious relationship between CEA content and me tumor cell differentiation. The positivity percentage for malignancy in gastric mucosal smears detected by CEAHRP-SPA technique was higher than that examined by histopathology.It is suggested that early stomach cancer could be determined and classified using CEA detection technique in combination with pathological examination.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1996年第9期647-650,共4页
Chinese Journal of Clinical Oncology
关键词
胃肿瘤
诊断
癌胚抗原
免疫组织化学
Carcinoembryonic antigen Stomach cancer Immunohistochemistry