鸡传染性法氏囊病毒的分离、纯化与抗血清的制备

Isolation and Purification of Infectious Bursal Disease Virus and Preparation of Antiserum

采用鸡胚尿囊液培养传染性法氏囊病毒（ＩＢＤＶ）。将培养病毒的鸡胚尿囊液经高速离心，聚乙二醇（ＰＥＧ）沉淀、蔗糖垫底超速离心、ＮａＢｒ密度梯度离心，可获得纯化的鸡传染性法氏囊病毒带。通过透射电子显微镜观察，表明ＩＢＤＶ颗粒呈六角形，直径约６０ｎｍ，无囊膜，其浮力密度为１．３０～１．３６ｇ／ｍｌ。实验所分离的病毒粒子的平均含量为１．７２ｍｇ／ｍｌ。用这种病毒免疫健康家兔制备抗ＩＢＤＶ的血清，获得了效价在１∶３２以上的抗血清。

To get pure infectious bursal disease virus (IBDV) should be done as followed: culture IBDV with allantoic fluid of chicken embryo, centrifuge the fluid at high speed, precipitate it with PEG, putting sucrose at the bottom to centrifuge it again at ultra-high speed, and at last do NaBr density gradient centrifugation. Under JEM-100CX, the virus particle was observed in six-angle shape, free from brain, with diameter being about 60 nm and the buoyant density 1.30-1.36 g/ml, the virus content averaged 1.72 mg/ml. Immuning this virus to rabbit can produce antiserum with 1∶32 of valence, showing high purity and specificity.