摘要
从黑龙江省哈尔滨地区田间采集典型小麦黄矮病株25份,用无毒麦二叉蚜(Shizaphis graminum)、麦长管蚜(Macrosiphum avenae)、禾谷缢管蚜(Rhopalosiphum-padi)传毒,经生物学分离并用BYDV—GAV、PAV、RPV、SGY四种抗血清进行酶联免疫吸附(即ELISA法)测定。结果表明:25份标样中由麦长管蚜(Ma)和麦二叉蚜(Sg)传播的有22份,与GAV抗血清有强反应,而与PAV、RPV、SGV抗血清无反应。表明GAV株系是哈尔滨地区大麦黄矮病毒的主要株系。
Samples of 25 plants of wheat with symptoms of barley yellow dwarf were collected from Harbin area, Heilongjiang province. Each sample was tested by enzyme-linked immunosor-bent assays (ELISA) using antisera for GAV,PAV,RPV,SGV and identifed by a series of biological experiments involving comparative transmission by Schizaphis graminum, Rhopalosiphum Padi and Macrosiphum avenae. Results indicated that 22 of 25 smples were transmitted non-specifically by S. avenae and S. graminum, and reacted very strongly with GAV-globulin and not at all with PAV,RPV and SGV globulins. These results pionted that GAV is the major strain of Barley Yellow Dwarf Virus in Harbin area.
出处
《黑龙江农业科学》
北大核心
1996年第6期1-3,共3页
Heilongjiang Agricultural Sciences
基金
"八六三"资助项目
