固定化辣根过氧化物酶在有机溶剂/水混合溶液中的电化学

Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture

用魔芋多糖(KGM)将辣根过氧化物酶(HRP)固定在玻碳电极(GCE)表面,制备了HRP-KGM膜修饰电极.在乙醇等亲水性有机溶剂与水的混合溶液中,包埋在KGM中的HRP可以与电极发生直接电子传递,且能催化还原过氧化氢、氢过氧化异丙基苯、氢过氧化叔丁基、过氧化丁酮等过氧化物.HRP-KGM膜修饰电极具有较好的稳定性和重现性,可用于这些物质的定量检测.

Horseradish peroxidase （HRP） was immobilized on glassy carbon electrode （CJCE） surface by konjac glucomannan （KGM）. HRP entrapped in the KGM film underwent fast direct transfer-electron reactions in water-organic solvent mixtures. The electrocatalysis of peroxides, such as hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, and 2-butanone peroxide, by the immobilized HRP was also explored. Stability and reproducibility of the modified electrode made it possible to determine the organic peroxides.