急性白血病儿童缓解前后血T淋巴细胞嗜银蛋白检测的意义

Significance of Determination of Argyrophilic Proteins in Nucleolar Organizer Regions of Blood T Lymphocytes before and after Complete Remission in Children with Acute Leukemia

目的通过对急性白血病(AL)儿童T淋巴细胞(T细胞)增殖活性的rDNA和rRNA的核仁组成区嗜银蛋白(Ag-NORs)测定,以了解患儿T细胞功能状态。方法分别对42例初发AL儿童化疗前与临床完全缓解后及30例正常儿童(对照组),用图像分析法测定血T细胞Ag-NORs(银染区)/细胞核面积(I.S%)。结果AL化疗前I.S%为5.06%±1.36%,与对照组7.51%±1.06%比较有显著差异(t=8.238 P<0.001),但在完全缓解后I.S%升至7.17%±0.98%,与化疗前比较(t=7.073 P<0.001);与对照组比较无差异(t=1.403 P>0.05)。结论在初发未化疗AL儿童中,Ag-NORs水平降低,提示患儿有T细胞免疫功能下降。

Objective To detect the argymphilic proteins in nucleolar organizer regions （Ag-NORs） that express rDNA and rRNA proliferation of T lymphocytes before chemotherapy and after complete remission （CR） in children with primary acute leukemia（AL）. Methods The argyrophilic granules area of NOR/nuclear area （I. S% ） of T lymphocytes was detected by image analysis system in peripheral blood of 42 patients before chemotherapy and after CR and 30 normal children. Results I. S% in the patients before chemotherapy（5.06%±1.36%） were signifieantly lower than those in the healthy donors（7,51%±1.06%） （t=8.238 P〈0.001）.I.S% in the patients after CR （7.17%±0.98% ） were significantly higher than those before chemotherapy （ t=7.073 P〈0.001）. However, no significant difference was encountered in I. S% between the patients after CR and the normal children （t=1.403 P〉0.05）. Conclusion These results suggest that decrease of Ag-NORs expresses the evidence for tumour induced suppression of immune function of T cells in children with AL prior to treatment.