人乳头状瘤病毒感染与食管癌发生关系的研究

Relationship between Human Papillomavirus Infection and the Development of Esophageal Carcinoma

为了探索人乳头状瘤病毒(HPV)与食管癌发生的关系,对食管鳞癌、癌旁鳞状上皮及乳头状瘤进行组织病理学研究,并用生物素标记的HPV_(16)DNA探针进行原位杂交。结果:癌旁鳞状上皮有凹空细胞等HPV感染的组织形态学改变者为65.2%(15/23),HPV_(160)DNA检出率为56.5%(13/23);食管鳞癌有凹空样癌细胞者为27.8%(10/36),HPV_(16)DNA检出率为50.0%(18/36);6例乳头状瘤均检出HPV_(16)DNA(100.0%)。不同分化程度的食管鳞癌(Ⅰ、Ⅱ、Ⅲ级)HPV_(16)DNA检出率分别为100.0%(6/6),60.0%(9/15)和20.0%(3/15)。研究结果提示,HPV感染与食管鳞癌发生有关,并与其分化和某些组织学特征有关。

Tissues of esophageal squamous cell carcinoma (ESQCC) ,papillo-ma and squamous epithelia adjacent to carcinoma (SEAC) were studied histopathologically and by in situ hybridization with HPV16DNA probe. Of the 23 cases studied 65. 2% (15/23) was found to display koilocytosis and in 56. 5% (13/23) HPV16 DNA was positive in SEAC. Koilocytic cancer cells were observed in 27.8% (10/36) of ESQCC and HPV16DNA was detected in 50. 0% (18/36) of ESQCC;HPV16DNA was detected in all 6 cases of papilloma (100. 0%). The detection rates of HPV16DNA in grade Ⅰ,Ⅱ,Ⅲ ESQCC were 100. 0% (6/6) ,60. 0% (9/ 15) ,and 20. 0% (3/15) respectively. The results of this study indicate that HPV infection is associated with the development,differentiation and some histologic characteristics of ESQCC.