摘要
采用Iodogen法将放射性131I与花生凝集素(PNA)进行标记,经腹腔注射131I-PNA标记物到荷人胃癌裸鼠体内,在不同时间测定组织放射分布和γ照相。给药后第3天,肿瘤组织放射性强度是血液的4.30倍,是正常肝组织的3.76倍,是肌肉组织的3.89倍;给药后第7天,肿瘤组织浓集131I-PNA是正常胃组织的4.21倍,是小肠组织的6.05倍,是大肠组织的5.13倍。48小时可见肿瘤显像,72小时显像清晰。本实验结果表明,凝集素PNA在胃癌定位诊断和导向治疗中有着潜在的应用价值,对于人体某些表达T抗原的腺癌都可能具有作为导向治疗载体的研究前景。
Peanut agglutinin (PNA) was Labelled with 131I by means of Iodogen method and was injected intraperitoneally into tumor bearing nude mice.The tissue distribution of 131I-labelled PNA was studied and whole body scintigraphies were made at different times. At the 3rd day after administration,the distribution ratio of tumor to normal tissue (T/NT) was 4.30 for blood, 3.76 for liver and 3.89 for the muscle.At the 7th day after administration, the tumor to normal stomach ratio was 4. 21,tumor to colorectum ratio was 5. 13. The tumors could be visualized on r-camera imaging at 48hr after administration and clear tumor Picture in all animals receiving 131I-PNA at 72hr.These results suggest that PNA has Potential value in localizing tumor and targeting therapy of human stomach cancer as a targeting vehicle.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1996年第12期889-892,共4页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金
关键词
胃肿瘤
腺癌
放射性核素显像
凝集素
碘131
Stomach neoplasm Radionuclide imaging Nude mice Lection 131I-labelled PNA