人醛缩酶A单抗ELISA对原发性肝细胞癌诊断价值的探讨

Diagnostic Value of Aldolase-A Determination by ELISA of Human Aldolase-A Monoclonal Antibody in Primary Hepatocellular Carcinoma

从人骨骼肌纯化醛缩酶（ＡＬＤ）Ａ，制备抗人ＡＬＤ－Ａ单克隆抗体，首次建立了测定血清ＡＬＤ－Ａ的竞争性ＥＬＩＳＡ，最小检出量０．１ｎｇ／ｍｌ，对ＡＬＤ－Ｂ和Ｃ无交叉反应。６０例正常人血清含量１３１±２４ｎｇ／ｍｌ，上限１７９ｎｇ／ｍｌ，均未超过此限；原发性肝细胞癌４５例５５４±１９３ｎｇ／ｍｌ，阳性率９５．５％（４４／４５），两组差异有非常显著意义（Ｐ＜０．００１）。对肝癌诊断有实用价值。单抗可大量制备，能使ＡＬＤ－Ａ检测标准化。

Aldolast-A (ALD-A) was purified from human skeletal muscle and then human ALD-A monoclonal antibody was prepared. A highly sensitive competitive ELISA was firstly established to determine the concentration of ALD-A in the serum. Minimal detectable concentration was 5ng/ml. Cross reactions with human ALD-B, C were not found. The serum levels of ALD-A in 60 normal subjects were 131±24 ng/ml. 95. 5% of 45 patients with primary hepatocellular carcinoma had ALD-A serum concentrtions above the cut-off level 179ng/ml. (P<0. 01). The determination of serum ALD-A by ELISA of human ALD-A McAb is thought to be valuable in the diagnosis of liver carcinoma.