肺癌层粘连蛋白表达及DNA含量与预后关系的研究

A Study on Laminin Expression and DNA Content in Lung Cancer and Its Relationship to Prognosis

对乙醇固定的３６例肺癌标本，采用ＰＡＰ法及流式细胞术（ＦＣＭ）进行层粘连蛋白（ＬａｍｉｎｉｎＬＭ）免疫组化测定和ＤＮＡ倍体及ＤＮＡ指数（ＤＩ）分析，并追踪随访６年。分析结果：ＬＭ阳性表达在组织学高分化组（１３／１９）高于低分化组（３／１７），二组间有显著性差异。也观察到同一病例不同部位ＬＭ表达有差异；本组异倍体率７７．１％（２７／３５），ＤＩ随癌组织分化程度的降低而增高；淋巴结转移组与无淋巴结转移组其ＬＭ表达及ＤＮＡ倍体分析未见显著性差异。随访结果：ＬＭ阳性组平均生存期（４１．４个月）明显高于ＬＭ阴性组（２８．１个月）（Ｐ＜０．０５）。５年以上生存组ＤＩ值１．２７９±０．１８明显低于５年以下组１．４１７±０．３２，二组有显著性差异（Ｐ＜０．００５）。作者认为：观察肺癌的ＬＭ表达与测定ＤＩ及ＤＮＡ倍体分析均有判断预后的意义。

Thirty-siX specimens of lung cancer fiXed with 95% alcohol were studied for Laminin(LM) expression and nuclear DNA content with PAP technic and flowcytometry.Thiry-two patients were followed up for more than 6 years.The results showed that there was significant difference in LM expression positivity between well and poorly differentiated groups,namely in 13 out of 19 cases in the for mer and 3 out of 17 cases in the latter. There were 27(77.1%) aneuploid and 8(22.9%)diploid.The DNA index(DI)increased with decrease of differentiation degree.No marked correlation was observed between the lymph node metastases and the LM expression or DNA content.The 5-year survival rate was 28.1%(9/32).Average survival period was 41.4 months in the LM positive group and 28. 1 months(P<0. 05) in the negative.DI was markedly lower in patients who survived over 5 years(1. 279±0.18)than in those who survived short of 5 years (1.417±0.32),the difference was significant statistically(P<0.005).These results suggest that observation of LM expression and DI in lung cancer may be helpful in assessing degree of malignancy and Prognosis.