五倍子水提取物对胶原酶活性的影响

The effect of the gallnut water extract on the activity of collagenase

目的:观察五倍子水提取物对牙周炎组织中胶原酶活性的影响,并探讨其抗炎作用机制。方法:应用羟脯氨酸检测法,观察6.25 ug/ml、12.5 ug/ml2、5 ug/ml、50 ug/ml、100 ug/ml 5种浓度五倍子水提取物对胶原酶活性的影响。结果:与胶原酶组比较,各浓度组五倍子水提取物均能明显降低胶原酶活性,其作用呈浓度依赖性。但浓度在100 ug/ml以内时,其抑制效果比5 ug/ml的强力霉素低。结论:五倍子水提取物能够阻断胶原酶对结缔组织的破坏作用,抑制或减缓牙周炎的进展。

Objective： To observe the effect of the gallnut water extract on the activity of collagenase. Method： hydroxyproline detection method was used to observe the effect of the gallnut water extract on the activity of collagenase. Resuit：The experiments showed that the gallnut water extract had a significant dose-dependent inhibitory effect on the activity of collagenase with a dose-dependent manner. However, the inhibitory effect of gallnut extract below 100 μg/ml was less than that of 5 〉g/ml doxycycline. Conclusion：The results indicated that gallnut water extract could block the connective tissues＇ destruction made by collagenase, thus inhibiting or lowering the progress of periodontal inflammation.