Y500R可吸收珊瑚羟基磷灰石人工骨体外成骨细胞相容性研究

Biocompatibility Study on osteogenesis of Y500R absorbable coral hydroxyapatite in vitro

目的:探讨Y500R可吸收珊瑚羟基磷灰石作为人工骨支架材料在体外的成骨效能,评价其组织相容性的优劣,为临床应用提供必要的实验依据。方法:采用酶消化法获得小鼠成骨细胞后,分为空白组、实验组和对照组。空白组为小鼠成骨细胞单独培养,实验组和对照组分别为颗粒型Y500R可吸收珊瑚羟基磷灰石、颗粒型羟基磷灰石(HA)与小鼠成骨细胞复合培养。对比小鼠成骨细胞在3组中的生长状况、形态学特征、碱性磷酸酶活性、矿化结节形成等成骨活性指标的差异,并采用扫描电镜观察、对比Y500R人工骨与角孔珊瑚在支架三维结构、孔隙率、孔径及孔的均匀度方面的差异。结果:实验组成骨细胞在成骨活性等指标方面与空白组无显著性差异。扫描电镜下可见Y500R可吸收珊瑚羟基磷灰石的断面呈多孔海绵状结构,微孔呈圆形,排列规则,与天然松质骨结构十分相似。Y500R的三维多孔结构和孔隙率与角孔珊瑚相比,结构类似,在电镜下几乎分不出差异,唯孔径比角孔珊瑚略小,但大小均匀、规则。结论:Y500R可吸收珊瑚羟基磷灰石具有良好的细胞相容性和成骨活性,复合培养不影响成骨细胞的正常生理功能,Y500R可吸收珊瑚羟基磷灰石能够满足组织工程对支架材料的要求,可作为一种新型的骨代用品。

Objective： To evaluate the effect of the osteogenesis of rat＇s osteoblasts cultured with Y500R absorbable coral hydroxyapatite in vitro and study the biocompatibility of the Y500R powder as a scaffolds in tissue engineering with the osteoblasts. Investigate the feasibility of the clinical use as a kind of inductional bone substitute in bone engineering. Method： Osteoblasts of rats obtained by enzymatic digestion were cultured and divides into 3groups： blank osteoblasts were cultured alone, study osteoblasts were seeded onto Y500R powder and the cell/Y500R composites were cultured in vitro, control osteoblasts were cultured with HA powder in vitro. Then the proliferation of the osteoblasts , ALP activity and protein concentration were measured, Y500R and HA powder were analyzed by scanning electron microscope to compare differences of the 3-D structure , porosity, and diameter of Y500R and HA powder porosity. Result： There were no significant difference in activity of ALP, proliferation, protein concentration between osteoblasts cultured with Y500R and those cultured alone. There were also no significant difference on physical structure between Y500R and nature coral analyzed by scanning electron microscope. Conclusion： It was suggested that Y500R absorbable coralline hydroxyapatite might have good cellular biocompatibility and might be used as a bone substitute on clinical use. It might be a good substitution of scaffold material for bone tissue engineering.