液相色谱-串联质谱法鉴定大鼠血浆中的山莨菪碱及其代谢物

Identification of Anisodamine and Its Metabolites in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry

目的液相色谱-电喷雾离子阱串联质谱(LC-MSn)联用法鉴别大鼠血浆中的山莨菪碱及其主要代谢物。方法取单剂量灌胃20 mg山莨菪碱的大鼠血样,甲醇沉淀蛋白,采用LC-MS及LC-MSn等方法分析血样。和空白血样及山莨菪碱相比较,根据血样中代谢物相对分子质量的变化(ΔM)及其多级质谱数据,鉴定并阐述其结构。结果在服药后的大鼠血样中发现6种代谢物,分别为6β-羟基托品、N-去甲基6β-羟基托品、脱水山莨菪碱、苯氧化山莨菪碱、N-氧化山莨菪碱以及托品酸。结论该方法灵敏,快速,简便,适合于药物及其代谢物的快速鉴定。

OBJECTIVE To identify anisodamine and its metabolites in rat plasma by combining chromatography and tandem mass spectrometry（LC-MSn）. METHODS Methanol was added to the plasma sample from rats after single administration of 20 mg anisedamine for precipitating protein, then the supematant was analyzed by LC-MSn. Identification and structural elucidation of the metabohtes were performed by comparing their changes in molecular masses （AM）, retentio n-times and full scan MSn spectra between the parent drug and blank plasma.RESULTS The results revealed that the parent drug and its six metabolites（ N-demethyl-6β-hydmxytropine, 6β-hydmxytropine, tropic acid, dehydrated anisedamine, hydmxyanisedamine, anisedamine N-oxide） existed in rat plasma. CONCLUSION This method is sensitive, rapid, simple, and it is suitable to the rapid identification of drug and its metabolits.