HPLC同时测定氢定乳膏中氢化可的松和醋酸氯己定的含量

Simultaneous Determination of Hydrocortisone and Chlohexidine Aacetate in Qingding Cream by HPLC

目的 建立测定氢定乳膏中氢化可的松和醋酸氯己定含量的方法。方法 采用高效液相色谱法。分析 色谱柱为Extend C18（4．6mm×150mm，5μm），流动相为甲醇-水相（醋酸铵26g和三乙胺37mL，加水至1000mL，冰醋酸调pH至4．0）（55：45）为流动相；流速为1．0mL·min^-1；检测波长为254nm；柱温为30℃；进样量为10止。结果 该方法不受处方中基质干扰，氢化可的松在0．1～0．6mg·L^-1内和醋酸氯己定在0．02～0．12mg·L^-1内，峰面积与质量浓度呈良好的线性关系（r hydrocortisone=0．9998，r chlohexidine acetate=0．9998），平均回收率：氢化可的松为99．6％、醋酸氯己定为99．2％。RSD分别为0．34％，1．41％（n=9）。结论 该方法可用于氢定乳膏中氢化可的松和醋酸氯己定的含量测定。

OBJECTIVE To develop a method for the determination of hydrocortisone and chlohexidine acetate in Qingding cream. METHODS A high-performance liquid chromatography method was used with Extend C18 column.The mobile phase consisted of methanolaqueous phase （ammonium acetate 26g and triethylamine 37 mL,imbitition to 1 000 mL, adjust pH to 4.0 by glacial acetic acid） （55：45） and the flow rate was 1.0 mL·min^-1, with the detection wavelength at 254nm.RESULTS The matrix component in cream didn＇t interfere with determination of hydrocortisone and chlohexidine acetate. The calibration curve was linear within the range of 0.1 - 0.6mg·L^-1 for hydrocortisone（ r = 0.999 8） and the range of 0.02 - 0.12mg·L^-1 for chlohexidine acetate（ r = 0.999 8） .The average recoveries of hydrocortisone and chlohexidine acetate were 99.6% and 99.2% respectively. RSD were 0.34% and 1.41% （ n = 9）, respectively. CONCLUSION The method can be used for the determination of hydrocortisone and chlohexidine acetate in Qingding Cream.