欧李茎尖培养脱毒研究

In vitro shoot tip tissue virus-free culture of Prunus humilis

以欧李嫩梢芽为外植体进行了微茎尖组培脱毒研究,探讨了在培养基中添加不同的激素成分及不同浓度对不定芽诱导、继代增殖和生根培养的影响。结果表明不定芽诱导的最佳培养基是:MS+6-BA0.4mg/l+IAA0.5mg/L;增殖培养的最适培养基为MS+6-BA0.3 mg/L+IAA0.4 mg/L;最佳生根培养基是:2/3MS+IAA0.5 mg/L。应用指示植物鉴定法进行病毒检测显示,0.3-0.4mm茎尖培养对苹果褪绿斑病毒(ACLSV)和李矮缩病毒(PDV)的脱毒率为71.6%和73%,实验证明是一种可靠实用的方法。

Buds or tender stem of Prunus humilis as explant, the micropropagation and the influence of the factors such as different hormone composition, concentration in culture medium upon adventitious bud induction, shoot proliferation and rooting were studied. The result shows that the optimum medium for adventitious bud regeneration is MS ＋ 6 - BA0.4 mg/L ＋ IAA0.5 mg/L; the best cuhure medium for shoot proliferation is MS ＋ 6 - BA0.3 mg/L ＋ IAA0.4 mg/L; the proper culture medium of rooting is 2/3MS ＋ IAA0.5 mg/L. The virus - free rate of ACLSV and PDV were 71.6% and 73% respectively on 0.3 - 0.4mm shoot tip based on the plant indicator identification, which is a reliable and adapt method.