摘要
目的:观察同型半胱氨酸对人血管内皮细胞组织因子mRNA(TFmRNA)表达的影响,探讨动脉粥样硬化发生的机制。方法:对照组常规血清培养,实验组以不同浓度的同型半胱氨酸培养实验用人血管内皮细胞,逆转录法检测其TFmRNA表达水平的变化。结果:对照组TFmRNA表达为0.86±0.21,添加同型半胱氨酸的分别为1.98±0.24,2.67±0.17,3.26±0.18,4.35±0.33,(P<0.05)。结论:同型半胱氨酸能促进人血管内皮细胞TFmRNA的表达。
Objective： To evaluate the effect of homocysteine（hcy） on the expression of TFmRNA in human vascular endothelial cells. Methods： The culture of human vascular endothelial cells was conducted with routine method in control group and with different concentration of homocysteine in experiment groups. The transcriptional level of TFmRNA was examined by RT-PCR. Results： The TFmRNA level in control group was lower （0. 86±0.21）, and the experiment levels of TFmRNA in four experiment groups were respectively 1. 98±0. 24,2. 67±0.17,3. 26±0. 18,4. 35±0.33, （P〈0.05）. Conclusion.. The homocysteine can enhance the expression of TFmRNA of human vascular endothelial cells.
出处
《医学理论与实践》
2006年第8期883-885,共3页
The Journal of Medical Theory and Practice
基金
深圳龙岗科技局课题,编号：200446.
