应用卵巢癌抗独特型单克隆抗体6B11进行血清中卵巢癌抗体检测

SerologicalAnalysisofAntibodiesbyAnti┐idiotypicMonoclonalAntibody6B11AgainstOvarianCar┐cinoma

目的：探讨应用卵巢癌抗独特型单克隆抗体６Ｂ１１进行血清中相关抗体检测的可行性。方法：采用间接免疫酶法测定妇科良性与恶性肿瘤患者各３０与８８例、子宫内膜异位症患者９例、正常女性献血员４５例共１７２份血清，１５例卵巢癌患者１年内１３３份血清中卵巢癌抗体水平，并与病情作比较。结果：以４５份正常血清卵巢癌抗体水平制定阈值，术前血清卵巢上皮癌患者卵巢癌抗体阳性率为６７．３％，明显高于子宫体恶性肿瘤及卵巢良性肿瘤患者（Ｐ＜０．００１）。１５例卵巢上皮癌患者一年内连续血清抗体检测结果与病情缓解有一定关系。结论：用卵巢癌抗独特型抗体检测血清中卵巢癌抗体水平，对卵巢癌诊断有一定辅助价值；并可作为监测指标之一。

Objective:Toestablishamethodfordetectionofserologicantibodybyanti-idiotypicmonoclonalantibody6B11againstovariancarcinoma.Method:Sera-antibodyexminationsfrom172cases,malignantandbenignovariananduterinetumorsaswelasnormalindividualsascontrolweredonerespectively.15casesofovarianepithelialcarcinomawith133bloodsampleswereexaminedse-rialybyenzymelinkedimmunoabsorbentassayduringthefirst12monthaftertreatment.Results:Thecut-ofvaluewasdeterminedandcalculatedfromseraofnormalcontrols.Thepositivityofep-ithelialovariancarcinomawas67.3%,muchhigherthanthatinthebenignovariantumorandmalig-nantorbenignuterinetumorswithstatisticalsignificantdiference.Theserialexaminationsoftheserain15casesofovarianepithelialcarcinomashowedthatthenegativeresultturnedfromtheposi-tiveonewascorrespondingwithdiseasestatus.Conclusions:Theexaminationofsera-antibodytest-edbyanti-idiotypicmonoclonalantibody6B11maygiveahelpforthediagnosisofovariancarcinomaandmaybeusedasoneofthetumormarkersforfolowup.