丁型肝炎抗原诊断试剂的制备研究

Preparation of diagnostic reagent for detecting hepatitis D antigen

采用从丁型肝炎患者血清中提取高效价的丁型肝炎抗体（抗－ＨＤ）及辣根过氧化物酶标法，制备丁型肝炎抗原（ＨＤＡｇ）诊断试剂，并对２２２例慢性乙型肝炎患者血清进行了检测。结果：本试剂与爱尔兰抗原试剂特异性比较无显著差异；抗体中和抑制率抗－ＨＤ为６９％～８５％，抗－ＨＢｃ为２４％～３３％，重复１０次检测其ＯＤ值的变异无显著性差异（Ｐ＞０．０５）；患者血清检测２２２例中，ＨＤＡｇ６例，阳性率为２．７％。提示：使用试剂操作简便，诊断快速，重复性好；其特异、敏感性与进口爱尔兰同类试剂比较结果基本一致。

The diagnostic reagent for detecting hepatitis D antigen(HDAg) was prepared from high titer antibody to HDAg(anti-HD) which was extracted from the sera of patients with hepatitis D and labelled with horseradish peroxidase. A total of 222 sera of patients withchronic hepatitis B was detected. The results showed that the specificity of the reagent had no difference from that of the antigen reagent of Ireland. The inhibitory percentages of neutralization with anti-HD and anti-HBc were respectively 69%～85% and 24%～33%, The distinction of OD values in ten times' repeated tests has no statistical significance(P>0.05). HDAg was found in six of the 222 sera of patients with chronic hepatitis B. The positive rate was 2.7%, It is suggested that the application of the reagent test samples may be convenient and rapid and may have a good repetition. The specificity and sensitivity of the reagent are identical with those of the antigen reagent of Ireland.