摘要
目的从遗传毒理学方面了解并评价纳米羟基磷灰石(nano-HA)人工骨的生物相溶性,从而为其在骨缺损修复中的临床运用提供依据。方法用纳米羟基磷灰石人工骨材料制备混悬液,进行Ames致突变试验,以检测其对鼠伤寒沙门菌的致突变比值(实验组回变菌落数/阴性对照组因变菌落数)。结果此人工骨材料的各浓度组混悬液对鼠伤寒沙门菌的致突变比值均小于2。结论纳米羟基磷灰石人工骨不引起鼠伤寒沙门菌的回复突变数增加,说明此纳米人工骨无致基因突变性。
Objective To evaluate the bio-compatibility and safety of nano-hydroxyapatite（nano-HA） artificial bone from the aspects of inherited toxicology of it for the clinical use of bone＇s reconstruction, Methods Produce the nano-hydroxyapatite artificial bone and it＇s diffusion and test the diffusion through Ames test. The observation markers of the test are the mutagens rates of murine tyhoid salmonella（MR）. Results The mutagenesis rates of routine typhoid saline mondlla in the experimental groups of all dosage levers are less than 2. Conclusion The nano-hydroxyapatite artificial bone will not cause the increase of the back mutation in murine typhoid salmonella which show that this artificiat bone will not cause gene mutation.
出处
《深圳中西医结合杂志》
2006年第4期193-195,共3页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
