胰岛素受体底物基因多态性与多囊卵巢综合征表型特征的关系

Relationship of Insulin Receptor Substrate Genotypes to Phenotypic Features of Polycystic Ovary Syndrome

目的评估胰岛素受体底物(IRS)蛋白,IRS-1(Gly972Arg)和IRS-2(Gly1057Asp)基因编码的多态性对非糖尿病多囊卵巢综合征(PCOS)患者胰岛素抵抗、糖耐量及雄激素水平的影响。方法用荧光定量聚合酶链反应(TaqManPCR)技术,检测62例非糖尿病多囊卵巢综合征(PCOS)患者IRS-1(Gly972Arg)和IRS-2(Gly1057Asp)基因编码的多态性,并测定血清睾酮以及75g葡萄糖负荷后患者的糖耐量、胰岛素水平。结果①拥有IRS-1基因型多态性的非糖尿病PCOS受试者在临床指标及激素测定差异无显著性(P>0.05)。②拥有IRS-2Gly/Gly基因型的非糖尿病PCOS受试者葡萄糖耐量试验(OGTT)2h血糖水平(8.1±0.4)mmol/L,高于Gly/Asp基因型的2h血糖水平(7.1±0.2)mmol/L和Asp/Asp基因型的2h血糖水平(6.7±0.2)mmol/L,差异有显著性(P<0.01)。而Gly/Gly基因型与Asp/Asp基因型之间差异差异无显著性(P>0.05)。结论IRS-2Gly1057Asp多态性影响非糖尿病PCOS患者血糖水平。因此,拥有IRS-2Gly/Gly基因型的个体可能具有更高的发展为Ⅱ型糖尿病的风险。

Objective To determine whether nondiabetic women with Polycystic Ovary Syndrome （PCOS） are more likely to have evidence of hyperandrogenism, glucose intolerance, or insulin resistance in the presence of the Gly^972Arg insulin receptor substrate （IRS）-1 and Gly^1057Asp IRS-2 polymorphisms. Methods 62 nondiabetic PCOS women were included. IRS-1 and IRS-2 Genotypes polymorphisms were detected with TaqMan polymerase chain reaction （TaqMan PCR）. The baseline parameters including levels of serum testosterone, oral glucose tolerance test （OGTT） and Insulin levels were measured. Results ①There was no association of IRS-1 genotype with any clinical or hormonal measure in nondiabetic PCOS subjects. ②Nondiabetic subjects with the IRS-2 Gly/Gly genotype had significantly higher 2-h oral glucose tolerance test glucose levels compared with those with Gly/Asp and Asp/Asp genotypes. Conclusion The IRS-2 Gly^1057Asp polymorphism influences blood glucose levels in nondiabetic women with PCOS.