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耐甲氧西林金葡球菌PBPs的研究方法 被引量:1

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摘要 描述了由我所在Sprat经典方法基础上加以改进的MRSAPBPs分离方法。包括:(1)细菌的培养;(2)膜蛋白的制备;(3)聚丙烯酰胺凝胶电泳;(4)放射自显影。
出处 《中国抗生素杂志》 CAS CSCD 北大核心 1996年第4期281-284,共4页 Chinese Journal of Antibiotics
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  • 1李栗,王浴生,雷幼导.放射性碘标记羟氨苄青霉素用于青霉素结合蛋白的研究[J].中国抗生素杂志,1993,18(1):47-49. 被引量:1
  • 2[4]Ubukata K, Yamashita N and Konno M. Occurrence of aβ-lactam-inducible penicillin-binding protein in methicillin-resistent Staphylococcus aureus. Antimicrob Agents Chemother, 1985, 27(5).851
  • 3[5]Chambers HF, Sachdeva MJ and Hackbarth CJ. Kinetics of penicillin binding to penicillin-binding proteins of Staphylococcus aureus. Biochem. J., 1994;301:139
  • 4[6]Chambers HF and Miick C. C haracterization of Penicillinbinding protein 2 of Staphylococcus aureus: deacylation reaction and identification of two penicillin-binding peptides. Antimicrob Agents Chemother, 1992; 36 (3): 656
  • 5[7]Utsui Y and Yokota T. Role of an altered penicillin-binding protein in methicllin-and cephem-resistant Staphylococcus aureus. Antimicrob Agents Chemother, 1985 ;28(3):397
  • 6[9]Higashi Y, Wakabayashi A, Matsumoto Y, et al. Role of inhibition of penicillin-binding proteins and cell wall cross-linking by beta-lactam antibioties in low-and high-level methicillin-resistance of Staphylococcus aureus. Chemotherapy, 1999; 45 ( 1 ): 37
  • 7[10]Pinho MG, de Lencastre H, Tomasz A. Cloning, characterization, and inactivation of the gene pbpC, encoding penicillin-binding protein 3 of Staphylococcus aureus. J. Bacteriol.,2000; 182(4): 1074
  • 8[11]Wada A, Watanabe H. Penidllin-binding protein 1 of Staphylococcus aureus is essential for growth. J. Bacteriol,1998; 180: 2759
  • 9[12]Pucci MJ, Thanassi JA, Discotto LF, et al. Identification and characterization of cell wall-cell division gene clusters in pathogenic gram-positive cocci. J. Bacteriol, 1997; 179: 5632
  • 10[13]Piniho MG, de Lencastre H, Tomasz A. Transcriptional analysis of the Staphylococcus aureus penicillin binding protein 2gene. J. Bacteriol, 1998; 180(23) :6077

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