采用GC法分析苦丁茶中可溶性单糖的研究

Determination of Soluble Monosaccharide in Zlex Lalifotia Thumb by GC

通过恒温水浸泡,干燥后利用二甲基亚砜(DMSO)作溶剂,六甲基二硅胺烷(TMDS)和三甲基氯硅烷(TMCS)作硅烷化试剂,将单糖衍生化为三甲基硅醚衍生物,以肌醇为内标物,用SE-30毛细管色谱柱,测定了苦丁茶中单糖的组成及含量。同时对比了二甲基酰胺及吡啶的溶解能力和N,O-双三甲基硅烷基乙酰基(BSA)的衍生化效果。方法的相对标准偏差小于8.0%,测得的苦丁茶中单糖含量分别为:葡萄糖0.355%,果糖0.440%,半乳糖4.000%,核糖0.035%,木糖0.150%,阿拉伯糖0.006%。

Monosaccharide in Kuding tea（ Zelex lalifotia thumb ） was obtained by dipping Kuding Tea in water bath with constant temperature, and then drying till the extract solution dryness. The dried monosaccharide was derived into trimethylsilyl derivative with hexamethyedisilane（HMDS） and trimethylchlorosilane（TMCS）, and dimethyl sulfoxide（DMSO） used as solvent. The monosaccharide was determined by GC with SE-30 capillary column, and myoinositol used as internal standard. The dissolving ability of dimethylformamide and pyridine were compared and the derivative effect of N, O-bitrimethylsilylacetamide （BSA） was determined. The relative standard deviations were below 8.0%. The contents of monosaccharides in Kuding tea were as follows： glucose（0.355%）, fructose（0.440%）, galactose（4.000%）, ribose（0.035%）, xylose（0.150%）, arabinose（0.006%）.