Alpha粒子和柴油机尾气颗粒提取物共同处理哺乳动物细胞的协同毒性作用

A Synergistic Toxic Effect in Mammalian Cells Induced by Co-treatment of Alpha Particle and Diesel Particulate Extract

为研究两种有害环境因子同时作用对人类健康的影响,以alpha粒子和柴油机尾气颗粒提取物(d iese lparticu late extract,DPE)共同处理皮肤成纤维细胞(AG1522)为实验模型,对细胞毒性、遗传毒性进行研究。实验发现和未处理的对照组相比,1 cGy alpha粒子和20μg/mL DPE处理24 h,共同作用于细胞能够导致细胞活力下降40%左右,微核形成率上升2倍多;而1 cGy alpha粒子单独辐照时细胞活力下降14.19%,微核形成率上升了36.84%,20μg/mL DPE单独处理24 h时细胞活力下降17.29%,微核形成率上升了73.68%。实验结果表明:这两种环境因子共同作用于哺乳动物细胞时,对于细胞的毒性不是简单的叠加,而是表现出一种协同效应。

In order to investigate the influence on the health of human beings of two environmentally detrimental factors working together, AG1522 cells co-treated by alpha particles and diesel particulate extract （DPE） were employed as an experimental model to explore their cytotoxicity and genotoxicity. It was found that, in comparison with control, a combination of 1 cGy alpha particle and 24-hour 20μg/mL DPE treatment led to a 40 percent reduction in cell viability and 2- fold increase in micronuclei formation, whereas either 1 cGy alpha particles radiation alone reduced cell viability by 14. 19 % and increased micronuclei formation by 36.84 % , or 24-hour 20 μg/mL DPE treatment alone diminished cell viability by 17.29 % and raised micronuclei formation by 73.68 %. Our current work demonstrates that the toxicity imposed on mammalian cells by these two environmental factors is not additive, but rather shows a synergistic effect.