核因子-κB和诱导型一氧化氮合酶在感染性休克大鼠肺组织中的表达及意义

Expression and significance of NF-κB and inducible nitric oxide synthase in lung tissue of rats with septic shock

目的:探讨感染性休克时大鼠肺组织内诱导型一氧化氮合酶(iNOS)和核因子-κB(NF-κB)活性变化的相关性,揭示其在感染性休克发病机制中的作用。方法:采用盲肠结扎穿孔术制备大鼠感染性休克模型,分别在术后1、6、12、24 h活杀大鼠取肺组织,用酶联免疫吸附法测定肺组织中膜NF-κB的活性,用RT-PCR和western-blot法检测肺组织中iNOS的表达。结果:感染性休克大鼠肺组织中膜NF-κB活性及iNOS mRNA和蛋白的表达均较对照组明显升高(P<0.01);NF-κB活性及iNOS mRNA和蛋白的表达呈时间依从性,术后1 h到达高峰,随后缓慢下降。结论:NF-κB是感染性休克发病机制上游的重要调控因子,其激活下游效应因子iNOS的表达是其参与感染性休克发病的重要途径。

Objective： To investigate the relationship between the expression of inducible nitric oxide synthase（iNOS） and the activity of nuclear factorκB（NK - κB） in lung tissue of septic rats, and to reveal their roles in mechanism of septic shock. Methods： The septic model of rat was established by cecumligation and puncture. The lungs were removed from the rats at 1, 6, 12 and 24 h after operation. The activity of NK - κB in lung tissue was detected by enzyme linked - immunoadsorbent assay（ELISA）, and the expression of iNOS was detected by RT - PCR and western blotting. Results：Compared with the control group, the activity of NK - κB and the expression iNOS increased in septic rats（P〈0. 01） with a time - dependence, they reached their peak values within 1 h and then decreased gradually. Conclusion： NK - κB is an important regulation factor for septic shock and it plays role in septic shock through activating the expression of its effector - iNOS.