苦瓜子叶节丛生芽的诱导

Induction of Multiple Buds from Cotyledonary Nodes of Balsam Pear (Momordica charantia L.)

以苦瓜(MomordicacharantiaL.)子叶节为材料,建立了苦瓜高效再生体系,并比较了不同基因型、不同激素浓度配比、苗龄和肌醇浓度对不定芽诱导率的影响。结果表明:子叶直立、略微分开,颜色呈浅绿色时子叶节诱导率最高;培养于MS+3.0mg/L6-BA+0.2mg/LIAA中的子叶节诱导率最高;不同基因型的诱导率有一定差异,以“南屿”苦瓜子叶节诱导率最高;添加一定浓度的肌醇可以促进不定芽的分化;低浓度6-BA的培养基有利于芽的伸长;低浓度IAA有利于不定根的发生,生根率高达91.7%。

Cotyledonary nodes of balsam pear （Momordica charantia L.） were. used for tissue culture to establish a high-frequency regeneration system, and the effect of different genotypes of Balsam pear, hormone combinations, seedling ages and concentrations of inositol were compared on the adventitious buds. The results showed that cotyledonary nodes gave the highest inducing frequency of multiple buds when the cotyledon is erect, slightly separate and pale green in color and cultured on the MS medium supplemented with 6-BA 3.0 mg/L ＋ IAA 0.2 mg/L. The inducing frequency varied with the genotypes, of which ＇Nanyu＇ was the highest. The inositol at a given concentration in the medium promoted the differentiation of the adventitious buds; the 6-BA was conducive to bud elongation at a low concentration; the IAA at low concentration favoured emergence of adventitious roots, resulting in a rooting rate of up to 91.7 %.