层粘连蛋白总糖肽对小鼠巨噬细胞功能的影响

The effects of laminin glycopeptides on murine macrophage functions

目的：观察层粘连蛋白总糖肽（Ｌａｍｉｎｉｎｇｌｙｃｏｐｅｐｔｉｄｅｓ，ＬＮ－Ｇｐｓ）对小鼠腹腔巨噬细胞（Ｍ）杀伤黑色素瘤Ｂ１６细胞及吞噬抗体包被的羊红细胞（ＥＡ）作用的影响。方法：将ＢＡＬＢ／ｃ小鼠腹腔Ｍ体外培养４８ｈ后，再分别与０（对照），５０，１００，２００μｇ／ｍｌ终浓度的ＬＮ－Ｇｐｓ预温３０ｍｉｎ，用噻唑蓝比色法检测Ｍ对Ｂ１６细胞的杀伤作用，用联苯胺氧化比色法检测Ｍ吞噬ＥＡ的功能。结果：（１）Ｍ杀伤Ｂ１６细胞的作用呈剂量依赖性增高，其中１００μｇ／ｍｌ和２００μｇ／ｍｌ的ＬＮ－Ｇｐｓ可分别使Ｍ对Ｂ１６细胞的杀伤作用较对照组增高（１０．３±２．８）％和（２８．４±９．９）％（Ｐ均＜０．０５）。（２）Ｍ对ＥＡ吞噬作用降低，分别为对照组的（９２．２±１２．４）％（ＬＮ－Ｇｐｓ５０μｇ／ｍｌ）、（８７．０±１４．２）％（ＬＮ－Ｇｐｓ１００μｇ／ｍｌ，Ｐ＜０．０５）和（６９．４±１８．２）％（ＬＮ－Ｇｐｓ２００μｇ／ｍｌ，Ｐ＜０．０５）。（３）如果将Ｂ１６细胞与ＬＮ－Ｇｐｓ２００μｇ／ｍｌ先预温，则Ｍ对Ｂ１６细胞的杀伤作用较对照组（Ｂ１６细胞不与ＬＮ－Ｇｐｓ预温）明显下降，为对照组的（８４．７±７．３）％（Ｐ?

Objective:To explore the effects of laminin glycopep des on the macrophage functions of cytotoxicity against B16 cells and phagocytosis of opsonized red cells.Methods:The macrophages,collected from BALB/c mouse peritoneal lumen, were cultured in vitro.After 48 h,LN-Gps were added at the final concentrations of 50, 100, and 200 μg/ml,37℃,30 min later,the M cytotoxicity to B16 target cell was measured by MTT colorimetric assay,and the M phagocytosis of EA was recorded using a microplate spectrophotometer.Results:(1) The macrophage cytotoxicity against B16 cells was enhanced as the levels of LN-Gps were elevated.When the macrophages were pre-exposed to 100 μg/ml and 200 μg/ml LN-Gps,their cytotoxicity against B16 cells were elevated by(10.3±2.8)%(P<0.05) and(28.4±9.9)%(P<0.05),respectively,as compared with the control.(2) The phagocytosis of opsonized red cells by macrophages decreased after the macrophages were pre-exposed to LN-Gps,the phagocytosis of treated groups were(92.2±12.4)% (LN-Gps 50 μg/ml),(87.0±14.2)% (LN-Gps 100 μg/ml,P<0.05) and(69.4±18.2)% (LN-Gps 200 μg/ml,P<0.05) of the control group.(3) If the B16 cells were pre-treated,while the macrophages were not treated with 2 0 0 μg/ml LN-Gps,the cytotoxicity of macrophage against B16 cells was significantly inhibited,only(84.7±7.3)% of the control group(P<0.05).Conclusion:The effects of laminin glycopeptides were not parallel in vitro.As to different cells,or to different functions of certain cells,the LN-Gps have different effects.The mechanisms are worth further studying.