野生与栽培管花肉苁蓉的遗传多样性分析

Study on genetic diversity of natural and cultivated Cistanche tubulosa

目的：研究野生与栽培管花肉苁蓉的遗传多样性。方法：采用随机扩增多态性DNA（RAPD）方法对管花肉苁蓉的4个野生居群和2个人工栽培居群的123个个体进行了遗传多样性研究。利用POPGENE1．31版软件分析群体的遗传多样性。结果：用10个随机引物共扩增出清晰谱带87条。野生居群平均多态位点百分率27．59，各居群多态位点百分率19．54～25．29，其中安迪尔居群的最高为25．29。2个人工栽培居群的多态位点百分率仅为13．79和11．49。用UPGMA法聚类可知，4个野生居群聚为一类，2个人工栽培居群聚为一类，表明野生居群与人工居群间已发生了遗传分化。结论：栽培管花肉苁蓉遗传背景单一，再次强调野生管花肉苁蓉保护的重要性。

Objective：To determine the genetic diversity of natural and cultivated Cistanche tubulosa.Method： 123 individuals of six populations of C. tubulosa, including four natural populations and two cultivated ones, were analyzed by random amplified polymorphic DNA （RAID） markers to determine the genetic variations among the populations. Result： A total of 87 loci （including 24 polymorphic loci） were amplified using 10 random primers. The average percentage d polymorphic loci （PPL） was 27.59 in the natural populations. The PPL between natural populations are 19.54 to 25.29. 0f the four natural populations, Andi＇er population had highest PPL （25.29）. The two cultivated populations had low PPL （13.79 and 11.49）. Cluster analysis using UPGMA revealed that populations of natural and cultivated were separated into two groups, the four natural populations clustered as one group and the two cultivated populations clustered as another group, indicating that the natural and cultivated populations had obvious differentiation. Conclusion： In view of the low genetic diversity of the cultivated C. tubulosa, we strongly suggested that the natural populations should be conserved in particular.