怀牛膝根成分的分离与鉴定

Isolation and identification of triterpenoids from root of Achyranthes bidentata in Henan

目的:研究怀牛膝Achyranthes bidentata根的三萜类化学成分。方法:利用凝胶、正相和反相硅胶色谱反复进行分离和纯化,通过理化、质谱和核磁共振波谱等技术鉴定化合物结构。结果:从怀牛膝根的水溶部分中分离得到7个化合物,分别鉴定为牛膝皂苷A(achyranthoside A,1)、牛膝皂苷E(achyranthoside E,2)、木鳖子皂苷Ib(mo-mordin Ib,3)、竹节参苷IVa(chikusetsusaponin IVa,4)、竹节参苷IVa甲酯(chikusetsusaponin IVa methyl ester,5)、竹节参苷V(chikusetsusaponin V,6)、竹节参苷V甲酯(chikusetsusaponin V methyl ester,7)。结论:化合物1和2为首次从该植物根中分离得到的天然化合物。

Objective： To investigate the triterpenoids from root of Achyranthes bidentata in Henan. Method： Sephadex, normal-and reversed-phase column chromatographies were applied for the isolation and purification. The structure determinations were performed by means of physiochemical properties, MS and NMR data analyses. Result： Seven compounds were isolated titan the water soluble fraction in toot of A. bidenture, and determined as achyranthoside A （1）, achyranthoside E （2）, momordin Ⅰb （3）, chikusetsusaponin Ⅳa （4）, chikusetsusaponin Ⅳa methyl ester （5）, chikusetsusaponin Ⅴ （6）, chikusetsusaponin Ⅴ methyl ester （7）. Conclusion：Compounds 1 and 2 were isolated titan the natural resources for the first time.