卷柏等5种中药植物雌激素活性筛选的实验研究

Experimental study of estrogenic activities of five kinds of Chinese herbal medicines

目的:对几种中药进行了植物雌激素的活性筛选。方法:采用动物实验结合报告基因技术,对5种中药进行了植物雌激素活性的研究。在体动物实验:采用出生21 d刚断乳昆明种雌性小鼠,随机分组,分别给予卷柏、松针、石胆草、贯众、益母草水提取物,阳性药为尼尔雌醇,持续给药7 d后处死小鼠,子宫称重,计算子宫系数;以雌激素应答元件(ERE)调控的报告基因瞬时表达检测,用荧光素酶检测试剂盒检测细胞上清液荧光素酶的活性,筛选出对α亚型雌激素受体(ERα)或β亚型雌激素受体(ERβ)有作用的中药。结果:卷柏提取物可使小鼠子宫重增加(P<0.01);且ERE调控的报告基因瞬时表达检测中,不论由ERα或ERβ介导均具有雌激素活性(P<0.01)。进一步子宫增重实验,对卷柏的有效部位进行了筛选,确定卷柏的有效部位为水部位和正丁醇部位。结论:卷柏及其水部位和正丁醇部位具有雌激素活性,且卷柏与ERβ的亲和力比与ERα的亲和力大,说明卷柏激活基因转录主要是通过ERβ介导的。

Objective： To evaluate the estrogenic activity of several kinds of Chinese herbal medicines. Method： Use zoopery and reporter gene technique to study the estrogenic activity of five Chinese herbal medicines. Zoopery： weanling female Kunming mice weight 9-12 g were administrated botanical extracts of Selaginllia tamariscina, Pinus Massoniana, Corallodiscus flabellate, Dryopteris sublaeta and Leonurus heterophyllus, the positive control group with Nilestriol tablets and control group with water, respectiely. On the eighth day, the animals were sacrificed and the uteri were separated solely and weighed. Reporter gene technique： Induce the expression of reporter gene controlled by ERE and measure the activity of luciferase on cell＇s clear supernatant. Result： The botanical extracts of S. tamariscina can increase the uterine weights of mice （ P 〈 0.01 ） ; In the expression of reporter gene controlled by ERE, Either ERα or EPβ＇s has estrogenic activity （ P 〈 0.01）. Follow in the zoopery we find the water part and the n-butanol part of S. tamariscina are the two active parts. Conclusion： S. tamarisina and it＇s water part and n-butanol part have estrogenic activities, effect on EPβ is greater than ERα.